Fig. 1

Localization of Rab4b wt and S22N. A EEA1, CI-M6PR, TGN46, p230, or GM130 labeling (red) in HeLa cells expressing the same amount of GFP-Rab4b (wt) or GFP-Rab4b S22N (green) shown as merged images. were treated for immunofluorescence using antibodies and anti-species Texas Red-coupled secondary antibodies. The bar corresponds to 10 µm. Enlarged views of the demarcated area are also shown. Arrowheads indicate to GFP-Rab4b-positive vesicles containing M6PR, whereas arrows point to Rab4b-positive structures not containing M6PR. B merged images of GFP-GOLPH (green) and HA-tag (red) labeling. The bar corresponds to 10 µm. Enlarged views of the delineated area are shown. C, D TEM images of GFP-Rab4b (10 nm gold particles) and TGN46 C or CI-M6PR D (15 nm gold particles) using mouse anti-GFP and rabbit anti-TGN46 C or rabbit anti-GFP and mouse anti-CI-M6PR (D). Enlarged views of the delineated area are shown and bars are for 500 nm