Fig. 3

Active Rab4b favors retrograde trafficking to TGN. A Western blot of sortilin on homogenates separated on a glycerol gradient prepared from HeLa cells or HeLa cells expressing GFP-Rab4b wt. Representative blots and the quantification of 4 independent experiments. * indicate that the differences are significant with p < 0.05 (One Way ANOVA) B, C Quantification of the percentage of iCD8-M6PR in the Golgi area labelled with GFP-GOLPH3 in single cells expressing HA-Rab4b wt B or HA-Rab4b Q67L C compared to the surrounding and non-transfected cells. Representative images are shown (left) using the protocol 1 described in Additional file 1: Fig. S2A. Red stars indicate the HA-Rab4b expressing cells. Red and yellow arrowheads point to the periphery of the cells in cells expressing or not-expressing Rab4b, respectively. *** indicates that the differences are significant with p < 0.0001 (student t test). The bars are for 10 µm