Fig. 6

VPS52, Rab4b and p230 localize to the CI-M6PR retrograde pathway. A confocal section taken at the center of cells (left panels) treated with the super-resolution algorithm SRRF. Individual channels were shown with p230 in blue, GFP-Rab4b in green, VPS52 in red, and internalized CI-M6PR (iCI-M6PR) in light grey as well as the merged image of p230, GFP-Rab4b, and VSP52 or of all the channels. Enlarged views of the periphery and Golgi areas are shown, the regions are marked by squares in the whole field of the merge of all colors. Arrows point to vesicles labelled by GFP-Rab4b, VPS52 and iCI-M6PR without p230, whereas the stars point to structures where all the labeling are close together. Bar represents 10 µm