Golgi-associated retrograde protein (GARP) complex-dependent endosomes to trans Golgi network retrograde trafficking is controlled by Rab4b

Table 1 List of antibodies and ligands

Protein	Reference	Application
CD8	Mouse monoclonal UCHT4, BIOMOL GmbH #153-020	Binding/IF
CI-M6PR	Mouse monoclonal MEM-238, EXBIO # 11-315-C100	IF/TEM/WB (non-reduced)
EEA1	Rabbit monoclonal C45B10, CST #3228	IF
EEA1	Mouse monoclanal, BD Biosciences #610456	IF
GFP	Mouse monoclonal 270F3, Synaptic Systems #132 011	IF/TEM
GFP	Polyclonal BD Living colors, BD Biosciences # 632460	IF/TEM
GM130	Mouse monoclonal, BD Biosciences #610822	IF
HA	Rabbit polyclonal, MBL #561	IF
HA	Mouse monoclonal HA.11, Covance #MMS-101P	IF/WB
c-Myc	Mouse monoclonal 9E10, Biolegend #62,801	WB
p230	Mouse monoclonal, BD Biosciences #611280	IF
Rab4b	Rabbit polyclonal anti mouse C-terminal Rab4b [40]	WB
Sortilin/NT3	mMuse monoclonal, BD Biosciences #612100	WB
TGN46/TGOLN2	Rabbit polyclonal, AbD serotec #AHP1586	IF/TEM
Alpha-tubulin	Mouse monoclonal, Sigma #56199	WB
VPS52	Rabbit poyclonal #HPA042667 Atlas Antibodies or gift [47]	WB
VPS52	Mouse polyclonal, Abnova #H00006293-B01	IF

ExBio, MBL (Clinisciences SAS, Nanterre, France); Cell Signaling technology, Atlas Antibodies (Ozyme Saint-Cyr-L’Ecole, France), BD Biosciences (Beckton Dickinson, Le Pont-de Claix, France), Synaptic Systems, Abnova (Interchim, Monluçon, France), Covance Antibody (Eurogentec, Seraing, Belgium), AbD Serotec (Bio-Rad, Marnes-La Coquette, France), Sigma-Aldrich (Saint Quentin Fallavier, France),, Biolegend Europe BV (Amsterdam, the Netherlands)
Alexa Fluor™-coupled anti-species and anti CI-M6PR (MEM) antibodies, cholera toxin, and transferrin are from Invitrogen (Fisher Scientific, IllKirch, France). DyLight™-coupled and HRP-coupled anti-species antibodies are from Jackson ImmunoResearch Laboratories (Cambridgeshire, UK). Gold-coupled anti species antibodies were from BBInternational (Cardiff, UK)

ISSN: 1689-1392